Calpastatin Peptide

Biochem/physiol Actions

Reversible: no

Product does not compete with ATP.

Cell permeable: yes

Target IC₅₀: 20 nM against calpain II

Primary Targetcalpain 2

General description

A 27-amino acid, peptide encoded by exon 1B of human calpastatin that acts as a cell-permeable and potent inhibitor of calpain I and calpain II (IC₅₀ = 20 nM for purified rabbit calpain II). Does not inhibit either papain or trypsin. Blocks the down-regulation of protein kinase C_ε (PKC_ε) in rat pituitary GH4C1 cells stimulated by thyrotropin-releasing hormone (TRH).

A 27-amino acid, cell-permeable peptide encoded by exon 1B of human calpastatin that acts as a potent inhibitor of calpain I and calpain II (IC₅₀ = 20 nM for purified rabbit calpain II). Does not inhibit either papain or trypsin. Blocks the down-regulation of protein kinase C_ε (PKC_ε) in rat pituitary GH₄C₁ cells stimulated by thyrotropin-releasing hormone (TRH).

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Eto, A., et al. 1995. J. Biol. Chem. 270, 25115.Kawasaki, J., et al. 1989. J. Biochem. 106, 274.Maki, M., et al. 1989. J. Biol. Chem. 264, 18866.

Packaging

500 µg in Glass bottle

Physical form

Supplied as a trifluoroacetate salt.

Reconstitution

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.

Sequence

Ac-Asp-Pro-Met-Ser-Ser-Thr-Tyr-Ile-Glu-Glu-Leu-Gly-Lys-Arg-Glu-Val-Thr-Ile-Pro-Pro-Lys-Tyr-Arg-Glu-Leu-Leu-Ala-NH₂

Warning

Toxicity: Standard Handling (A)